| StepH | Home | HS | AS | Away | StepA | Pens | Upset |
|---|---|---|---|---|---|---|---|
| Mon, Sep 16, 2024 | |||||||
| 3 | Redditch United | 0 | 1 | Shifnal Town | 5 | Yes | |
| 3 | Barwell | 1 | 2 | Buxton | 2 | ||
| Tue, Sep 17, 2024 | |||||||
| 2 | Scarborough Athletic | 5 | 2 | Dunston UTS | 4 | ||
| 2 | Scunthorpe United | 5 | 0 | Newcastle Town | 4 | ||
| 2 | Alfreton Town | 0 | 0 | Spalding United | 3 | 4-3 | |
| 2 | Slough Town | 2 | 1 | Chichester City | 3 | ||
| 3 | Warrington Rylands 1906 | 3 | 1 | Newton Aycliffe | 4 | ||
| 3 | Ilkeston Town | 0 | 1 | Hereford | 2 | ||
| 3 | Leiston | 3 | 1 | Brentwood Town | 4 | ||
| 3 | Wingate & Finchley | 0 | 1 | Cray Wanderers | 3 | ||
| 3 | Hungerford Town | 0 | 1 | Winchester City | 3 | ||
| 3 | Banbury United | 2 | 3 | Melksham Town | 4 | Yes | |
| 3 | Gloucester City | 0 | 2 | Gosport Borough | 3 | ||
| 4 | Hednesford Town | 4 | 1 | Rugby Town | 4 | ||
| 4 | Witham Town | 1 | 1 | Haringey Borough | 4 | 3-4 | |
| 5 | Barking | 0 | 1 | Gorleston | 4 | ||